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Study of Enzymatic Hydrolysis of Bacterial Nanocellulose

Received: 28 June 2014     Accepted: 10 July 2014     Published: 13 July 2014
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Abstract

Bacterial nanocellulose is a subject of extensive research due to its promising potential applications in medicine, veterinary and cosmetics. In this paper, effect of structural characteristics on enzymatic hydrolysis of bacterial nanocellulose (BNC) and microcrystalline cellulose (MCC) has been studied. Despite the similar degree of crystallinity and lateral size of crystallites for both cellulose samples, a conversion degree of BNC after enzymatic hydrolysis was considerably higher than of MCC. The main distinctive feature of the BNC sample is a high porosity and developed surface of nanofibrils. As against, the MCC sample contains coarse low-porous particles that have a poorly developed surface. Although drying reduces the porosity of the samples, the dry BNC retains a much higher pore volume and greater enzymatic hydrolysability than the dry MCC. Due to highly porosity and developed surface, the BNC sample acquires a high accessibility for molecules of cellulolytic enzymes that promotes enzymatic hydrolysis of this sample both in never-dried and dry state.

Published in American Journal of BioScience (Volume 2, Issue 6-1)

This article belongs to the Special Issue Chemical Biology

DOI 10.11648/j.ajbio.s.2014020601.13
Page(s) 13-16
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Bacterial Nanocellulose, Crystallinity, Porosity, Specific Surface, Enzymatic Hydrolysis

References
[1] Klemm D., Schumann D., Kramer F., Hessler N., Hornung M., Schmauder H-P., and Marsch S. “Nanocelluloses as innovative polymers in research and application,” Adv. Polym. Sci., vol. 205, pp. 49-96, 2006.
[2] Hubbe M.A., Rojas O.J., Lucia L.A., and Sain M. “Cellulosic nanocomposites – a review,” Bioresources, vol. 3, pp. 929-980, 2008.
[3] Habibi Y., Lucia L.A., and Rojas O.J. “Cellulose nanocrystals chemistry, self-assembly, and applications,” Chem. Rev., vol. 110, pp. 3479–3500, 2010.
[4] Ioelovich M. “Peculiarities of cellulose nanoparticles,” TAPPI, vol. 13, pp. 45-52, 2014.
[5] Klemm D., Schumann D., Kramer F., Heßler N., Koth D., and Sultanova B. “Nanocellulose materials – different cellulose, different functionality,” Macromol. Symp., vol. 280, pp. 60-71, 2009.
[6] Klemm D., Heublein B., Fink H-P., and Bohm A. “Cellulose: fascinating biopolymer and sustainable raw material,” Angew. Chem., vol. 44, pp. 2-37, 2005.
[7] Ioelovich M. Cellulose – Nanostructured Natural Polymer. Saarbrucken: Lambert, 2014.
[8] Ioelovich M., and Morag E. “Effect of cellulose structure on enzymatic hydrolysis,” Bioresources, vol. 6, pp. 2818-2834, 2011.
[9] Ioelovich, M. “Supermolecular structure of native and iso-lated cellulose,” Acta Polymerica, vol. 43, pp. 110-113, 1992.
[10] Ioelovich M., Leykin A., and Figovsky O. “Study of cellulose paracrystallinity,” Bioresources, vol. 5, pp. 1393–1407, 2010.
[11] Jayme G., and Hahn G. “A simple method of WRV-measuring for celluloses,” Papier, vol.14, pp. 138-139, 1960.
[12] Jayme G., and Büttel H. “Concerning the determination and the meaning of water retention value (WRV) of various bleached and unbleached cellulosic pulps,” Wochenbl. Papiefabr., vol. 96, pp. 180-187, (1968).
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    Michael Ioelovich. (2014). Study of Enzymatic Hydrolysis of Bacterial Nanocellulose. American Journal of BioScience, 2(6-1), 13-16. https://doi.org/10.11648/j.ajbio.s.2014020601.13

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    ACS Style

    Michael Ioelovich. Study of Enzymatic Hydrolysis of Bacterial Nanocellulose. Am. J. BioScience 2014, 2(6-1), 13-16. doi: 10.11648/j.ajbio.s.2014020601.13

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    AMA Style

    Michael Ioelovich. Study of Enzymatic Hydrolysis of Bacterial Nanocellulose. Am J BioScience. 2014;2(6-1):13-16. doi: 10.11648/j.ajbio.s.2014020601.13

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  • @article{10.11648/j.ajbio.s.2014020601.13,
      author = {Michael Ioelovich},
      title = {Study of Enzymatic Hydrolysis of Bacterial Nanocellulose},
      journal = {American Journal of BioScience},
      volume = {2},
      number = {6-1},
      pages = {13-16},
      doi = {10.11648/j.ajbio.s.2014020601.13},
      url = {https://doi.org/10.11648/j.ajbio.s.2014020601.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.s.2014020601.13},
      abstract = {Bacterial nanocellulose is a subject of extensive research due to its promising potential applications in medicine, veterinary and cosmetics. In this paper, effect of structural characteristics on enzymatic hydrolysis of bacterial nanocellulose (BNC) and microcrystalline cellulose (MCC) has been studied. Despite the similar degree of crystallinity and lateral size of crystallites for both cellulose samples, a conversion degree of BNC after enzymatic hydrolysis was considerably higher than of MCC. The main distinctive feature of the BNC sample is a high porosity and developed surface of nanofibrils. As against, the MCC sample contains coarse low-porous particles that have a poorly developed surface. Although drying reduces the porosity of the samples, the dry BNC retains a much higher pore volume and greater enzymatic hydrolysability than the dry MCC. Due to highly porosity and developed surface, the BNC sample acquires a high accessibility for molecules of cellulolytic enzymes that promotes enzymatic hydrolysis of this sample both in never-dried and dry state.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Study of Enzymatic Hydrolysis of Bacterial Nanocellulose
    AU  - Michael Ioelovich
    Y1  - 2014/07/13
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajbio.s.2014020601.13
    DO  - 10.11648/j.ajbio.s.2014020601.13
    T2  - American Journal of BioScience
    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
    SP  - 13
    EP  - 16
    PB  - Science Publishing Group
    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.s.2014020601.13
    AB  - Bacterial nanocellulose is a subject of extensive research due to its promising potential applications in medicine, veterinary and cosmetics. In this paper, effect of structural characteristics on enzymatic hydrolysis of bacterial nanocellulose (BNC) and microcrystalline cellulose (MCC) has been studied. Despite the similar degree of crystallinity and lateral size of crystallites for both cellulose samples, a conversion degree of BNC after enzymatic hydrolysis was considerably higher than of MCC. The main distinctive feature of the BNC sample is a high porosity and developed surface of nanofibrils. As against, the MCC sample contains coarse low-porous particles that have a poorly developed surface. Although drying reduces the porosity of the samples, the dry BNC retains a much higher pore volume and greater enzymatic hydrolysability than the dry MCC. Due to highly porosity and developed surface, the BNC sample acquires a high accessibility for molecules of cellulolytic enzymes that promotes enzymatic hydrolysis of this sample both in never-dried and dry state.
    VL  - 2
    IS  - 6-1
    ER  - 

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  • Designer Energy Ltd, Rehovot, Israel

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